High-throughput culture and embedment of spheroid array using droplet contact-based spheroid transfer
نویسندگان
چکیده
منابع مشابه
Reversibly-assebmled Perfusion Culture Chip with Microwell Array for Controllable Spheroid Culture and Post-culture Analysis
This paper reports a microfluidic device composed of a reversibly-assembled microfluidic chamber chip and multi-microwell array chip. This device enabled perfusion culture of spheroid in a microarray format, and detailed post-culture analysis by collection of spheroids from the disassembled device. The device provides four advantages simultaneously; the uniform spheroid formation, growth analys...
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Background: Radiotherapy is an established therapeutic modality for prostate cancer. Resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. No data is available on the response of cellular spheroid to Reseveratol. In this study we have examined the effect of Resveratol on the radiation response of human p...
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We describe and share a device, methodology and image analysis algorithms, which allow up to 66 spheroids to be arranged into a gel-based array directly from a culture plate for downstream processing and analysis. Compared to processing individual samples, the technique uses 11-fold less reagents, saves time and enables automated imaging. To illustrate the power of the technology, we showcase a...
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ژورنال
عنوان ژورنال: Biomicrofluidics
سال: 2018
ISSN: 1932-1058
DOI: 10.1063/1.5039965